Step 1. | Use a micropipette to dispense 900 mL of the diluent to glass tube. |
Step 2. | Use a micropipette to transfer 100 mL of the test solution to the first well. Discard the tip. |
Step 3. | Mix by shaking by hand or using a vortex mixer. |
Step 4. | The well now contains 100 mL of the original test solution diluted by one tenth in a total volume of 1000 mL. |
Step 1. | Set up the sterilized glass test tubes in a rack. Label each tube clearly to indicate the dilution of its contents after the ten-fold serial dilution has been carried out. |
Step 2. | Use a micropipette to dispense 900 mL of the diluent to all the labeled sterile tubes. |
Step 3. | Use a micropipette to transfer 100 mL of the test solution to the first tube and mix. This is the first ten-fold dilution. |
Step 4. | Use a micropipette with new sterile tip to carry out a second ten-fold dilution. |
Step 5. | Continue the series of ten-fold dilutions until the last tube. |